A77-01 (607737-87-1) Description:
A-77-01 is a potent inhibitor of the TGF-β1 receptor ALK5 (IC50 = 25 nM).It has no effects on BMP-regulated transcriptional activity or MAPK pathways. A-77-01 efficiently prevents the growth inhibitory effects of TGF-β on lung epithelial Mv1Lu cells. It completely blocks phosphorylation of SMAD2 by TGF-β in human keratinocyte HaCaT cells at 1 µM. A-77-01 prevents morphological and gene expression changes characteristic of epithelial-to-mesenchymal transition in NMuMG cells treated with TGF-β.
Target: ALK5 A-77-01 is a close analogue of A-83-01(Axon 1421) and has a very similar biological profile of A-83-01. A-83-01 is found to decompose to A-77-01 under certain circumstances and A-77-01 is likely an active component or metabolite of its prodrug A-83-01.
A77-01 (607737-87-1) Specifications:
|Synonyms||A 77-01; A-77-01.|
|Molecular Weight||286.338 g/mol|
|Monoisotopic Mass||286.122 g/mol|
|Solubility||Soluble in DMSO (20 mg/ml), and ethanol (20 mg/ml).|
|Storage Temp.||0 – 4 C for short term (days to weeks), or -20 C for long term (months).|
|Shelf life||>2 years if stored properly|
|Handling||Protect from air and light|
|Application||An inhibitor of ALK5 with an IC50 of 25nM|