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PI3K&mTOR

3-Methyladenine (3-MA) (5142-23-4)

3-Methyladenine is an inhibitor of class III phosphatidylinositol 3-kinase (PI 3-kinase).It can protect cerebellar granule cells from apoptosis following serum/potassium deprivation. 3-Methyladenine inhibits phosphoinositide 3-kinase (PI3K) activity, which plays a key role in autophagy process.

Not Intended for Therapeutic Use. For research use only.

CAS: 5142-23-4 Category

3-Methyladenine (3-MA) (5142-23-4) Description:

3-Methyladenine, aslo known as NSC 66389,is a selective PI3K inhibitor for Vps34 and PI3Kγ, blocks class I PI3K consistently, whereas suppression of class III PI3K is transient, and also suppress the formation autophagosome. 3-methyladenine enhances the sensitivity of nasopharyngeal carcinoma cells to chemotherapy and radiotherapy. 3-Methyladenine can depress drug efflux transporters via blocking the PI3K-AKT-mTOR pathway thus sensitizing MDR cancer to chemotherapy. 3-methyladenine potentiates apoptosis induced by dietary tocotrienols in breast cancer cells. 3-MA inhibits autophagy by blocking autophagosome formation via the inhibition of type III Phosphatidylinositol 3-kinases (PI-3K). For use as an autophagy inhibitor, 3-MA is typically used at a concentration of 5 mM. In studies investigating N-methylations of DNA bases, use of deuterated methylating agents allows for monitoring of alkylation patterns with mass spectrometry. 3-Methyl-d3-adenine is potentially useful as a reference sample for identifying adenine alkylations in such studies.

 

3-Methyladenine (3-MA) (5142-23-4) Specifications:

Product Name 3-Methyladenine (3-MA)
Synonyms 3-Methyladenine, NSC 66389; NSC-66389; NSC66389
Chemical Names 3-methyl-3H-purin-6-amine; 3-MA; 6-Amino-3-methylpurine; AK-94088; J-640198; W-202935; NSC66389
Purity ≥98% (HPLC)
CAS Number 5142-23-4
Molecular Formula C6H7N5
Molecular Weight 149.15
Monoisotopic Mass 149.07 g/mol
MDL number MFCD03701198
InChIKey ZPBYVFQJHWLTFB-UHFFFAOYSA-N
InChi Code InChI=1S/C6H7N5/c1-11-3-10-5(7)4-6(11)9-2-8-4/h2-3,7H,1H3,(H,8,9)
SMILES CN1C=NC(=N)C2=C1N=CN2
Form Powder
Color White to faint yellow
Solubility  Soluble in DMSO (10 mg/ml) or 100% ethanol (5 mg/ml, warm).
Storage Temp.  Store at +4°C
Shelf life >2 years if stored properly
Handling Protect from light and moisture
Application A multi-use inhibitor that protects cerebellar granule cells from apoptosis

 


RIDADR NONH for all modes of transport

References:

[1]. Slavin SA et al. Am J Physiol Lung Cell Mol Physiol. Autophagy inhibitor 3-methyladenine protects against endothelial cell barrier dysfunction in acute lung injury. DOI: 10.1152/ajplung.00555.2016.  PMID: 29074492

[2]. Harroun SG et al. Spectrochim Acta A Mol Biomol Spectrosc.Biomarkers of cigarette smoking and DNA methylating agents: Raman, SERS and DFT study of 3-methyladenine and 7-methyladenine. DOI: 10.1016/j.saa.2016.12.012   PMID: 28061366

[3]. Cheng NT et al. Int J Mol Med. Role of autophagy in the progression of osteoarthritis: The autophagy inhibitor, 3-methyladenine, aggravates the severity of experimental osteoarthritis.  DOI: 10.3892/ijmm.2017.2934  PMID: 28339018

[4]. Song L, Ma L, Chen G, Huang Y, Sun X, Jiang C, Liu H. [Autophagy inhibitor 3-methyladenine enhances the sensitivity of nasopharyngeal carcinoma cells to chemotherapy and radiotherapy]. Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2016 Jan;41(1):9-18. doi: 10.11817/j.issn.1672-7347.2016.01.002. Chinese. PubMed PMID: 26819419.

[5]. Hermann R, Vélez DE, Rusiecki TM, Fernández Pazos Mde L, Mestre Cordero VE, Marina Prendes MG, Perazzo Rossini JC, Savino EA, Varela A. Effects of 3-methyladenine on isolated left atria subjected to simulated ischaemia-reperfusion. Clin Exp Pharmacol Physiol. 2015 Jan;42(1):41-51. doi: 10.1111/1440-1681.12323. PubMed PMID: 25311855.