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Monosialotetrahexosylganglioside Sodium (GM1) pig brain (37758-47-7)

Monosialotetrahexosylganglioside Sodium (GM1) pig brainpig brain is a member of the ganglio series of gangliosides that contain one sialic acid residue, also known as GM1, with potential neuroprotective and neuroregenerative activities. It also acts as the site of binding for both cholera toxin and E. coli heat-labile enterotoxin…

CAS: 37758-47-7 Category

Monosialotetrahexosylganglioside Sodium (GM1) pig brain (37758-47-7) Description:

Monosialotetrahexosylganglioside Sodium (GM1) pig brain extracted from pig brain. Useful as an antigen and receptor for cholera toxin, as a growth inhibition marker in fibroblasts, and as a marker for lymphoid subpopulations. Monosialotetrahexosylganglioside Sodium, one of the glycosphingolipids widely distributed in all tissues, occurs in highest concentrations in the central nervous system. It is primarily located in the outer surface of the mammalian cell’s plasma membrane and in synaptic membranes of the CNS. Monosialotetrahexosylganglioside Sodium modulates a number of cell surface and receptor activities as well as neuronal differentiation and development, protein phosphorylation and synaptic function. In addition, it exerts anti-excitotoxic activity, prevents necrosis, and improves neuronal recovery and function. Check for active clinical trials using this agent.

Monosialotetrahexosylganglioside Sodium (GM1) pig brain (37758-47-7) Specifications:

Product Name Ganglioside GT1b
Synonyms Ganglioside G1 Mixture
Chemical Names Ganglioside GT1b (d18:1/14:0)
Purity ≥98% (HPLC)
CAS Number 59247-13-1
Molecular Formula C95H162N5O47 • 3Na (for Sphingosine C18:1)
Molecular Weight 2195.0
Monoisotopic Mass 2195.7
MDL number MFCD00081622
InChIKey XWMACUIMZLECSZ-IXGHXQDLSA-K
InChi Code InChI=1S/C95H165N5O47.3Na/c1-7-9-11-13-15-17-19-21-22-24-26-28-30-32-34-36-64(118)100-52(53(112)35-33-31-29-27-25-23-20-18-16-14-12-10-8-2)47-134-87-75(125)74(124)78(62(45-106)137-87)139-89-77(127)85(147-95(92(132)133)39-56(115)67(98-50(5)110)83(145-
SMILES CCCCCCCCCCCCC/C=C/[[email protected]]([H])([[email protected]](NC(CCCCCCCCCCCCCCCCC)=O)([H])CO[[email protected]]1[[email protected]@H]([[email protected]]([[email protected]@H]([[email protected]](O1)CO)O[[email protected]@H]2O[[email protected]@H]([[email protected]@H]([[email protected]@H]([[email protected]]2O)O[[email protected]@]3(C([O-])=O)O[[email protected]]([[email protected]@H]([[email protected]](C3)O)NC(C)=O)[[email protected]@H](O)[[email protected]](O[[email protected]@]4(C([O-])=O)O[[email protected]]([[email protected]@H]([[email protected]](C4)O)N
Form Powder
Color White or off-white
Solubility  /
Storage Temp.  −20°C
Shelf life >2 years if stored properly.
Handling Protect from air and light
Application /
Character White or white powder; slightly smelly, tasteless; wet. This product is soluble in water, very slightly dissolved in methanol, almost insoluble in anhydrous ethanol.
Identification
Color rendering reaction 1 Take the test solution 0.2ml under the content determination, add water 2ml, and then add the Hydroquinone hydrochloric acid solution (hydroquinone 0.2g, add water 10ml dissolved, add hydrochloric acid 90ml, add 0.1mol/L Copper sulfate solution 0.25ml, refrigerator Preservation) 2ml, water bath heating for 15 minutes, solution blue and purple, with N-e alcohol 5ml Vibration extraction, n-e alcohol layer is blue.
Color rendering reaction 2 Take this product about 20mg, add glacial acetic acid 1ml, hot water bath heating fully dissolved, add three ferric chloride test fluid 1 drops, in the hot water bath along the pipe wall slowly add sulfuric acid 1ml, so that the solution into two layers, the two-liquid interface should be brown
Sodium salt The identify reaction of sodium salt in this product (Chinese Pharmacopoeia 2015 edition four 0301).
Liquid chromatography(LC) In the chromatographic graph recorded under the content determination, the retention time of the main peak of the test solution should be consistent with the retention time of the main peak of the control solution.
Infrared spectroscopy The infrared light absorption map of this product should be consistent with the map of the control product (Chinese Pharmacopoeia 2015 edition four 0402).
Inspect
PH 5.0~6.5
Clarity and color of solution Should be clarified colorless; if it is cloudy, it shall not be thicker than the standard liquid of turbidity No. 1th; If the color rendering should not be deeper than the Yellow No. 1th colorimetric solution.
High Pressure determination The finished solution should be clear and transparent after high temperature, no milk light, no precipitation
Sensitizing factor (Miscellaneous protein) ≤50000
Total Sialic acid According to the dry product calculation, the saliva containing acid should be 19.0~21.0%
Related substances Sialic acid ≤0.3%;GD3, GD1a, unknown impurities are ≤ 0.5%; impurities and ≤2.0%
Protein ≤1.0%
High molecular weight impurities ≤0.5%
Residual solvents Methanol ≤ 0.1%; acetone ≤ 0.3%; Trichloromethane ≤0.003%
Water ≤4.0%
Burning residue ≤5.0%
Heavy metal ≤20ppm
Abnormal toxicity Comply Regulations
Antihypertensive substances Should be in accordance with the regulations (multiple quantities of administration)
Pyrogen Should be in accordance with the regulations (multiple quantities of administration)
Microbial limits The total number of aerobic bacteria should be <100cfu/g, and the total number of molds and yeasts should be <50cfu/g

 


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RIDADR NONH for all modes of transport

References:

[1]. Protective effects of monosialotetrahexosylganglioside sodium on H2O2-induced human vascular endothelial cells. Zhao H, Li X, Li G, Sun BO, Ren L, Zhao C. Exp Ther Med. 2015 Sep;10(3):947-953. Epub 2015 Jun 30. PMID: 26622420

[2]. Cerebral trauma, Campylobacter jejuni infection, and monosialotetrahexosylganglioside sodium mediated Guillain-Barré syndrome in a Chinese patient: a rare case event. Zhang GZ, Li XG. J Neuropsychiatry Clin Neurosci. 2014 Apr 1;26(2):E16-7. doi: 10.1176/appi.neuropsych.13030073. No abstract available. PMID: 24763774

[3]. Ganglioside GM1 promotes contact inhibition of growth by regulating the localization of epidermal growth factor receptor from glycosphingolipid-enriched microdomain to caveolae. Zhuo D, Guan F. Cell Prolif. 2019 May 24:e12639. doi: 10.1111/cpr.12639. [Epub ahead of print], PMID: 31127673

[4].Pathophysiology of Ganglioside GM1 in Ischemic Stroke: Ganglioside GM1: A Critical Review. Zhang W, Krafft PR, Wang T, Zhang JH, Li L, Tang J. Cell Transplant. 2019 Jan 22:963689718822782. doi: 10.1177/0963689718822782. [Epub ahead of print], PMID: 30666888