DAPT (GSI-IX) (208255-80-5) Description:
DAPT, also known GSI-IX, is a potent and selective γ-secretase inhibitor. DAPT is a non-transition-state analogue GSI and is the compound most widely evaluated in experimental models. DAPT causes a reduction in Aβ levels in human primary neuronal cell cultures with IC50 values = 115−200 nM. DAPT blocks Notch signaling in a cell-based assay measuring the activation of the Notch pathway reporter gene with an IC50 = 500 nM. As an inhibitor of Notch, DAPT has also been used in the study of autoimmune and lymphoproliferative diseases.
DAPT is a γ-secretase inhibitor and indirectly an inhibitor of Notch, a γ-secretase substrate. Other γ-secretase substrates include LDL receptor-related protein, E-cadherin and ErbB-4. As an inhibitor of γ-secretase, DAPT may be useful in the study of β-amyloid (Aβ) formation. DAPT has been shown to inhibit Notch signaling in studies of autoimmune and lymphoproliferative diseases, such as ALPS and lupus erythematosus (SLE), as well as in cancer cell growth, angiogenesis, and differentiation of human induced pluripotent stem cells (hIPSC).
DAPT (GSI-IX) (208255-80-5) Specifications:
|Synonyms||DAPT;DAPT peptide; GSI-IX; GSI IX; N-(2FPhAc)Ala-phenyl-Gly t-butyl ester.|
|Chemical Names||tert-butyl (S)-2-((S)-2-(2-(3,5-difluorophenyl)acetamido)propanamido)-2-phenylacetate|
|Purity||>98% (or refer to the Certificate of Analysis)|
|Monoisotopic Mass||432.186 g/mol|
|SMILES||O=C(OC(C)(C)C)[[email protected]](C1=CC=CC=C1)NC([[email protected]](C)NC(CC2=CC(F)=CC(F)=C2)=O)=O|
|Solubility||Soluble in DMSO, not in water|
|Storage Temp.||Dry, dark and at 0 – 4 C for short term (days to weeks) or -20 C for long term (months to years).|
|Shelf life||>2 years if stored properly|
|Handling||Protect from air and light|
|Application||DAPT is a γ-secretase inhibitor with IC50s of 115 and 200 nM for total Aβ and Aβ42, respectively.|